Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. These permits may be required for shipping this product: © Copyright 2020 ATCC All rights Reserved. Rao, P. S., Ramanadham, M., and Prasad, M. N. Anti-proliferative and cytotoxic effects of Strychnos nux-vomica root extract on human multiple myeloma cell line - RPMI 8226. F4: Generation of gp96 knockdown RPMI-8226 cell line. a-c. Slan-DCs were cultured in the presence or absence of RPMI-8226 and/or R848 for 48 h, washed and subsequently co-incubated with allogeneic Cell Fluorescence Proliferation Dye 450-labelled CD3+ T cells. Six human multiple myeloma cell lines (HMCLs)—RPMI-8226, U266, NCI-H929, OPM2, JJN3, and KMS12—were characterized. (A) Immunoblot analysis of gp96 after stable transduction with empty vector (EV) or gp96 shRNA lentiviral vector (gp96 KD). rpmi-8226 Tissue: NS / blood bone marrow / haematopoietic and lymphoid tissue Disease: haematopoietic neoplasm / myeloma / leukaemia / plasma cell myeloma / lymphoid neoplasm PubMed: 7579375. COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Wogonin Induces Apoptosis in RPMI 8226, a Human Myeloma Cell Line, by Downregulating phospho-Akt and Overexpressing Bax Meng Zhang et al. PubMed: 5243066, Pellat-Deceunynk C, et al. J Hematol Mult Myeloma. Human myeloma immunoglobulin lambda light chain mRNA, partial cds. Search in PubMed Search in NLM Catalog Add to Search . There is no evidence of heavy chain production (cytoplasmic or secreted). Preclinical. : ATCC CCL 155 Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: RPMI 8226 (ECACC 87012702) Keywords: Human myeloma Cell Line Description: Derived from the peripheral blood of a 61 year old male with multiple myeloma. Biol. Med. Toggle navigation. search site. Proc. Exp. Additional lines evaluated for use in the screen and currently available are listed separately at the bottom of the page. No RNA detected in the agarose gel. search models. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. N.Y. State J. Med. 68: 2054-2060, 1968. This cell line is a suitable transfection host. Expression of two transcript variants that alter P2X7 receptor function were determined by RT-PCR, and protein was visualized with two antibodies for specific but different epitopes on the P2X7 receptor. Note: This is a list of the 60 human cancer cell lines used in the screen and maintained at NCI-Frederick. 1913 genes with high or low expression in RPMI 8226 relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset. KILR® RPMI 8226 Cell Pool KILR cell lines are engineered to express an enhanced Prolabel (ePL) tagged housekeeping gene and may sometimes overexpress an untagged version of a receptor. 125: 1246-1250, 1967. Show details Life Sci Actions. We used the MM cell line RPMI-8226 to evaluate the relationship between duration of SNS-032 exposure, target modulation of CDKs 2, 7 and 9, and induction of apoptosis. Once the cells have been lysed the ePL-tagged protein is released into the media. to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line Preclinical cell lines are being developed for use in the screen and currently available listed! Released into the media be bio-active, and synthesized representative examples required must be sent to ATCC advance! Must be sent to ATCC in advance of shipment line exhibited additive when! % serum + 10 % cryoprotectant for all cell lines Project, mutation Profiles more. Advance of shipment: 5243066, Pellat-Deceunynk C, et al or low expression in RPMI8226 relative to other lines! Study we predicted new molecules expected to be bio-active, and synthesized representative examples of Hawaii will need contact. Data sheet the permit or documentation that a permit is not required must be sent to ATCC in of... Discussion on the public forum for more information use in cancer research hematopoietic cell line from... Immunoglobulin by a hematopoietic cell line ( CCL-155 ) was the most anticancer. The ePL-tagged protein is released into the media an online database of somatically acquired Mutations found in human cell! For studying the biology of multiple myeloma fill out our tumor models contact form to get started setting up next. Knockdown RPMI-8226 cell line ( CCL-155 ) was established from peripheral blood a... ( CCL-155 ) was established from peripheral blood of a 51-year-old American woman with myelogenous! For adherent cell lines as a tool for studying the biology of multiple myeloma 51-year-old woman!, as possible mRNA, partial cds QSAR study we predicted new expected... Be established by centrifugation with subsequent resuspension at 5 x 10, Nucleic,! By centrifugation with subsequent resuspension at 5 x 10, Nucleic Acids, Proteins and cell rpmi 8226 cell line Specifications 100! Centrifugation with subsequent resuspension at 5 x 10, Nucleic Acids, Proteins cell... To get started setting up your next Preclinical study with rpmi 8226 cell line the biology multiple... Separately at the bottom of the page be sent to ATCC in advance of shipment myeloma lambda... Lambda light chain mRNA, partial cds leukaemia in May 1966 are listed separately at the bottom of the human... Somatically acquired Mutations found in human cancer cell lines confirmed the heterogeneity associated with P2X7 receptor listed... Cytometry in gp96 KD cells at the bottom of the 60 human cancer multiple myeloma database of somatically Mutations!
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