•1 OD260 Unit = 40ug/ml for single stranded RNA A password reset email has been sent to the primary email address associated with your account. Please update your browser to Internet Explorer 11 or above. Calculate plasmid DNA concentration from a260 values Online tools to calculate DNA properties, DNA solution concentration, dilution and absorbance. Note that the calculator will give weird results if your concentration is zero (it will ask for infinite amounts of DNA), or if it’s lower than the desired final concentration (it will ask for negative amounts of water). dsDNA: µg to pmol Online DNA calculator of DNA solution and DNA dilution Use the following formula to estimate your DNA: Concentration (ug/ml) = A260 reading x dilution factor x 50 ug/ml. DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. Please contact Customer Service to unlock your account. Please try again or contact Customer Service. MGE Innovation Center 505 South Rosa Road, Suite 238 | Madison, WI 53719. To dilute a solution of concentrated acid or base of known w/w% strength, please use the Acid & Base Molarity Calculator. Also calculate molarity of solutions, perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs. The concentration of any nucleotide can be calculated by inputting the nucleic acid sequence e: Extinction coefficient of DNA, RNA or oligoes, which is as follows: Please try again or contact Customer Service. Congratulations! To calculate the Molarity of a 70 wt. Nano drop concentration of all the four samples, 1. Stay notified of Promega events, products and news. This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μL. Just use undiluted stock DNA in these cases. •1 OD260 Unit = 50ug/ml for double stranded DNA Thank you for verifying your email address. •1 OD260 Unit = 20ug/ml for single stranded oligo (ssOligo) The concentration is expressed at 70% wt./wt. If you know that the weight of your DNA (molar mass per bp) is different from the average weight of DNA basepair, change the value. l: width of the cuvette which used to hold the solution, in cm, usually is 1cm. Typically, qPCR instrument software will perform the necessary plotting and calculations for concentration determinations. Then, using the A260 reading, you can calculate the DNA concentration. This calculation is based on the assumption that the average weight of a base pair (bp) is 650 Daltons. This calculator is used to determine the concentration of DNA solutions using an absorbance reading at 260 nm. Choose a DNA, RNA, genome editing, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry.
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